TyrRS rabbit pAb
TyrRS rabbit pAb from ELK Biotechnology Read more ›
Product Description
TyrRS rabbit pAb is an ELK Biotechnology antibody for research workflows involving TyrRS.
Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | TyrRS Polyclonal Antibody detects endogenous levels of TyrRS |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela cells using TyrRS Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 |
Key Facts
| Target | TyrRS |
| Also known as | Tyrosine--tRNA ligase, cytoplasmic (EC 6.1.1.1) (Tyrosyl-tRNA synthetase) (TyrRS) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000,IHC 1:50-300 ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 60kD |
| Cellular localization | Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Aminoacyl-tRNA biosynthesis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000,IHC 1:50-300 ELISA 1:10000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human YARS. AA range:451-500 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.