ABM99827
ETBR rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ETBR rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
ETBR rabbit pAb is an ELK Biotechnology antibody for research workflows involving ETBR.
Background
The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016],
Additional antibody information
| Specificity | ETBR Polyclonal Antibody detects endogenous levels of ETBR |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded Human placenta. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
Product type
Primary Antibody›
Target: ETBR
| Target | ETBR |
| Also known as | Endothelin B receptor (ET-B;ET-BR;Endothelin receptor non-selective type) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 50kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein. internalized after activation by endothelins. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>Neuroactive ligand-receptor interaction;>>Melanogenesis;>>Relaxin signaling pathway;>>Pathways in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | Synthesized peptide derived from ETBR at AA range: 31-80 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p: 100-300.WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
ETBR
Also known as
Endothelin B receptor (ET-B;ET-BR;Endothelin receptor non-selective type)
Concentration
1 mg/ml
Working concentration
IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
Synthesized peptide derived from ETBR at AA range: 31-80
Observed band
50kD
Cellular localization
Cell membrane ; Multi-pass membrane protein. internalized after activation by endothelins. .
Dilution range
IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed