Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99817

GABARAP rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GABARAP rabbit pAb from ELK Biotechnology Read more ›

GABARAP rabbit pAb
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GABARAP rabbit pAb

Product Description

GABARAP rabbit pAb is a research-use antibody from ELK Biotechnology for studying GABARAP in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MAP1 LC3 family.,subunit:Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin (By similarity). Interacts with ULK1.,tissue specificity:Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.,

Additional antibody information

Specificity GABARAP Polyclonal Antibody detects endogenous levels of GABARAP
Validation evidence captions Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: GABARAP
Collaborator ELK Biotechnology
Partner model ES8482
Target GABARAP
Research areas>>FoxO signaling pathway, >>Autophagy - other, >>Mitophagy - animal, >>Autophagy - animal, >>NOD-like receptor signaling pathway, >>GABAergic synapse
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of GABARAP AA range: 26-75
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GABARAP
Also known as
GABA(A) receptor-associated protein-like 2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthetic Peptide of GABARAP AA range: 26-75
Observed band
17kD
Cellular localization
Cytoplasmic vesicle, autophagosome . Endoplasmic reticulum membrane . Golgi apparatus .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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