Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99814

Hamartin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Hamartin rabbit pAb from ELK Biotechnology Read more ›

Hamartin rabbit pAb
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Hamartin rabbit pAb

Product Description

Hamartin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Hamartin in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009],

Additional antibody information

Specificity Hamartin Polyclonal Antibody detects endogenous levels of Hamartin
Validation evidence captions Western Blot analysis of 3T3 cells using Hamartin Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: Hamartin
Collaborator ELK Biotechnology
Partner model ES8478
Target Hamartin
Research areas>>Phospholipase D signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Cellular senescence, >>Thermogenesis, >>Insulin signaling pathway, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Herpes simplex virus 1 infection, >>Choline metabolism in cancer
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Hamartin . at AA range: 360-440
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Hamartin
Also known as
tuberous sclerosis 1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Hamartin . at AA range: 360-440
Observed band
130kD
Cellular localization
Cytoplasm . Membrane ; Peripheral membrane protein . At steady state found in association with membranes. .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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