TLE1/2/3/4 rabbit pAb from ELK Biotechnology Read more ›
TLE1/2/3/4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TLE1/2/3/4 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.,PTM:Phosphorylated, probably by CDC2. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.,similarity:Belongs to the WD repeat Groucho/TLE family.,similarity:Contains 6 WD repeats.,subcellular location:Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.,subunit:Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif).,tissue specificity:In all tissues examined, mostly in brain, liver and muscle.,
| Specificity | TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4 |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 3T3 mouse-liver SH-SY5Y K562 Hela 293T cells using TLE1/2/3/4 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8464 |
| Target | TLE1/2/3/4 |
| Research areas | >>Wnt signaling pathway, >>Notch signaling pathway |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4. AA range:721-770 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | In all tissues examined, mostly in brain, liver and muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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