E2F-3 (Acetyl Lys168) rabbit pAb
E2F-3 (Acetyl Lys168) rabbit pAb from ELK Biotechnology Read more ›
Product Description
E2F-3 (Acetyl Lys168) rabbit pAb is an ELK Biotechnology antibody for research workflows involving E2F-3.
Background
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013],
Additional antibody information
| Specificity | This antibody detects endogenous levels of E2F-3 at Human:K168;Mouse:K160;Rat:K161, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | E2F-3 |
| Also known as | E2F3 KIAA0075 |
| Concentration | 1 mg/ml |
| Working concentration | WB: 1:500-10000 ELISA: 1:10000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 50kD |
| Cellular localization | Nucleus. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Endocrine resistance;>>Cell cycle;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Immunogen | Synthesized acetyl-peptide from human protein at AA range: 130-200 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.