E2F-3 (Acetyl Lys168) rabbit pAb from ELK Biotechnology Read more ›
E2F-3 (Acetyl Lys168) rabbit pAb is a research-use antibody from ELK Biotechnology for studying E2F-3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013],
| Specificity | This antibody detects endogenous levels of E2F-3 at Human:K168;Mouse:K160;Rat:K161, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8447 |
| Target | E2F-3 |
| Research areas | >>Endocrine resistance, >>Cell cycle, >>Cellular senescence, >>Cushing syndrome, >>Hepatitis C, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human T-cell leukemia virus 1 infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Pancreatic cancer, >>Glioma, >>Prostate cancer, >>Melanoma, >>Bladder cancer, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:500-10000 ELISA: 1:10000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized acetyl-peptide from human protein at AA range: 130-200 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bone marrow,PCR rescued clones,Placenta,Pre-B cell,Rectum tumor,Uterus, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:500-10000 ELISA: 1:10000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
