ABM99756
FAT10 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
FAT10 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
FAT10 rabbit pAb is an ELK Biotechnology antibody for research workflows involving FAT10.
Background
similarity:Contains 2 ubiquitin-like domains.,subunit:Interacts with MAD2.,
Additional antibody information
| Specificity | FAT10 Polyclonal Antibody detects endogenous levels of FAT10 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of K562 293T using FAT10 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of K562 using FAT10 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
Product type
Primary Antibody›
Target: FAT10
| Target | FAT10 |
| Also known as | UBD; FAT10; Ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 18kD |
| Cellular localization | Nucleus . Cytoplasm . Accumulates in aggresomes under proteasome inhibition conditions. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from the Internal region of human FAT10. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
FAT10
Also known as
UBD; FAT10; Ubiquitin D; Diubiquitin; Ubiquitin-like protein FAT10
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from the Internal region of human FAT10.
Observed band
18kD
Cellular localization
Nucleus . Cytoplasm . Accumulates in aggresomes under proteasome inhibition conditions.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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