Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99746

Tensin-2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Tensin-2 rabbit pAb from ELK Biotechnology Read more ›

Tensin-2 rabbit pAb
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Tensin-2 rabbit pAb

Product Description

Tensin-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Tensin-2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the tensin family. Tensin is a focal adhesion molecule that binds to actin filaments and participates in signaling pathways. This protein plays a role in regulating cell migration. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Tensin-2 Polyclonal Antibody detects endogenous levels of Tensin-2 protein.
Validation evidence captions Western blot analysis of A549 3T3 using Tensin-2 antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from HepG2 cells, using Tensin-2 antibody.

Key Facts

Product typePrimary Antibody Target: Tensin-2
Collaborator ELK Biotechnology
Partner model ES8345
Target Tensin-2
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human Tensin-2. AA range:439-488
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in heart, kidney, brain, thymus, spleen, liver, placenta, lung, skeletal muscle and small intestine.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Tensin-2
Also known as
TENC1; KIAA1075; TNS2; Tensin-like C1 domain-containing phosphatase; C1 domain-containing phosphatase and tensin homolog; C1-TEN; Tensin-2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Tensin-2. AA range:439-488
Observed band
125kD
Cellular localization
Cell junction, focal adhesion . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Detected at the end of actin stress fibers. Detected in cytoplasmic punctate bodies (PubMed:22019427). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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