ABM99738
NICE4 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
NICE4 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
NICE4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving NICE4.
Background
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 UBA domain.,tissue specificity:Ubiquitous.,
Additional antibody information
| Specificity | NICE4 Polyclonal Antibody detects endogenous levels of NICE4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of NIH-3T3 K562 NIH-3T3 293T cells using NICE4 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of HEPG2 using NICE4 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 and 293 cells, using UBAP2L Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using UBAP2L antibody. |
Key Facts
Product type
Primary Antibody›
Target: NICE4
| Target | NICE4 |
| Also known as | UBAP2L; KIAA0144; NICE4; Ubiquitin-associated protein 2-like; Protein NICE-4 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 115kD |
| Cellular localization | Cytoplasm, Stress granule . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human UBAP2L. AA range:561-610 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
NICE4
Also known as
UBAP2L; KIAA0144; NICE4; Ubiquitin-associated protein 2-like; Protein NICE-4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human UBAP2L. AA range:561-610
Observed band
115kD
Cellular localization
Cytoplasm, Stress granule .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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