NICE4 rabbit pAb from ELK Biotechnology Read more ›
NICE4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NICE4 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 UBA domain.,tissue specificity:Ubiquitous.,
| Specificity | NICE4 Polyclonal Antibody detects endogenous levels of NICE4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of NIH-3T3 K562 NIH-3T3 293T cells using NICE4 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of HEPG2 using NICE4 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT-29 and 293 cells, using UBAP2L Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using UBAP2L antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8122 |
| Target | NICE4 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human UBAP2L. AA range:561-610 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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