TBC1D4 (phospho Thr642) rabbit pAb
TBC1D4 (phospho Thr642) rabbit pAb from ELK Biotechnology Read more ›
Product Description
TBC1D4 (phospho Thr642) rabbit pAb is an ELK Biotechnology antibody for research workflows involving TBC1D4.
Background
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Ph
Additional antibody information
| Specificity | Phospho-TBC1D4 (T642) Polyclonal Antibody detects endogenous levels of TBC1D4 protein only when phosphorylated at T642. |
|---|---|
| Validation evidence captions | Western blot analysis of L929 using p-TBC1D4 (T642) antibody. Antibody was diluted at 1:2000 | Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide. |
Key Facts
| Target | TBC1D4 |
| Also known as | TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160 |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 150kD |
| Cellular localization | Cytoplasm . Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Thyroid hormone signaling pathway;>>Insulin resistance;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642. AA range:611-660 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.