p164-RhoGEF rabbit pAb from ELK Biotechnology Read more ›
p164-RhoGEF rabbit pAb is a research-use antibody from ELK Biotechnology for studying p164-RhoGEF in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases.,similarity:Contains 1 DH (DBL-homology) domain.,tissue specificity:Highly expressed in the heart.,
| Specificity | p164-RhoGEF Polyclonal Antibody detects endogenous levels of p164-RhoGEF protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HepG2 cells, using CARD6 Antibody diluted at 1:50. The picture on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-human heart. 1, Antibody was diluted at 1:200(4° overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8102 |
| Target | p164-RhoGEF |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF17. AA range:431-480 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in the heart. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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