MSK1 (phospho Ser360) rabbit pAb from ELK Biotechnology Read more ›
MSK1 (phospho Ser360) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MSK1 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
| Specificity | Phospho-MSK1 (S360) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S360. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MSK1 (Phospho-Ser360) Antibody | Immunofluorescence analysis of NIH/3T3 cells, using MSK1 (Phospho-Ser360) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser360) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of MSK1 (Phospho-Ser360) Antibody. The lane on the right is blocked with the MSK1 (Phospho-Ser360) peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8000 |
| Target | MSK1 |
| Research areas | >>MAPK signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>TNF signaling pathway, >>Circadian entrainment, >>Neurotrophin signaling pathway, >>Shigellosis, >>Pathways in cancer, >>MicroRNAs in cancer, >>Bladder cancer |
| Reactivity | Human,Mouse |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Ser360. AA range:331-380 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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