Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99679

ACTR-IIA rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ACTR-IIA rabbit pAb from ELK Biotechnology Read more ›

ACTR-IIA rabbit pAb
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ACTR-IIA rabbit pAb

Product Description

ACTR-IIA rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACTR-IIA in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013],

Additional antibody information

Specificity ACTR-IIA Polyclonal Antibody detects endogenous levels of ACTR-IIA protein.
Validation evidence captions Western blot analysis of lysates from rat kidney, using ACTR-IIA antibody.

Key Facts

Product typePrimary Antibody Target: ACTR-IIA
Collaborator ELK Biotechnology
Partner model ES7986
Target ACTR-IIA
Research areas>>Cytokine-cytokine receptor interaction, >>TGF-beta signaling pathway, >>Signaling pathways regulating pluripotency of stem cells, >>Fluid shear stress and atherosclerosis
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ACTR-IIA. AA range:10-59
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Mammary gland,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ACTR-IIA
Also known as
ACVR2A; ACVR2; Activin receptor type-2A; Activin receptor type IIA; ACTR-IIA; ACTRIIA
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ACTR-IIA. AA range:10-59
Observed band
48kD
Cellular localization
Membrane; Single-pass type I membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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