ABM99619
cPLA2-γ rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
cPLA2-γ rabbit pAb from ELK Biotechnology Read more ›
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Product Description
cPLA2-γ rabbit pAb is an ELK Biotechnology antibody for research workflows involving cPLA2-γ.
Background
This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009],
Additional antibody information
| Specificity | cPLA2-γ Polyclonal Antibody detects endogenous levels of cPLA2-γ protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat cells, using PLA2G4C Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using PLA2G4C antibody. |
Key Facts
Product type
Primary Antibody›
Target: cPLA2-γ
| Target | cPLA2-γ |
| Also known as | PLA2G4C; Cytosolic phospholipase A2 gamma; cPLA2-gamma; Phospholipase A2 group IVC |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 70kD |
| Cellular localization | Cell membrane ; Lipid-anchor . Endoplasmic reticulum membrane ; Lipid-anchor. Mitochondrion membrane ; Lipid-anchor. Lipid droplet . Translocates from endoplasmic reticulum to lipid droplets in response to oleate. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Arachidonic acid metabolism;>>Linoleic acid metabolism;>>alpha-Linolenic acid metabolism;>>Metabolic pathways;>>MAPK signaling pathway;>>Ras signaling pathway;>>Phospholipase D signaling pathway;>>Necroptosis;>>Vascular smooth muscle contraction;>>VEGF signaling pathway;>>Platelet activation;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Glutamatergic synapse;>>Serotonergic synapse;>>Long-term depression;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Ovarian steroidogenesis;>>Oxytocin signaling pathway;>>Choline metabolism in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLA2G4C. AA range:301-350 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
cPLA2-γ
Also known as
PLA2G4C; Cytosolic phospholipase A2 gamma; cPLA2-gamma; Phospholipase A2 group IVC
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PLA2G4C. AA range:301-350
Observed band
70kD
Cellular localization
Cell membrane ; Lipid-anchor . Endoplasmic reticulum membrane ; Lipid-anchor. Mitochondrion membrane ; Lipid-anchor. Lipid droplet . Translocates from endoplasmic reticulum to lipid droplets in response to oleate. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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