Caveolin-2 (phospho Tyr27) rabbit pAb from ELK Biotechnology Read more ›
Caveolin-2 (phospho Tyr27) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Caveolin-2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. This gene and related family member (CAV1) are located next to each other on chromosome 7, and express colocalizing proteins that form a stable hetero-oligomeric complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Additional isoforms resulting from the use of alternate in-frame translation initiation codons have also been described, and shown to have preferential localization in the cell (PMID:11238462). [provided by RefSeq, May 2011],
| Specificity | Phospho-Caveolin-2 (Y27) Polyclonal Antibody detects endogenous levels of Caveolin-2 protein only when phosphorylated at Y27. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 5' , using Caveolin 2 (Phospho-Tyr27) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7828 |
| Target | Caveolin-2 |
| Research areas | >>Endocytosis, >>Focal adhesion, >>Prion disease, >>Bacterial invasion of epithelial cells, >>Proteoglycans in cancer, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caveolin 2 around the phosphorylation site of Tyr27. AA range:12-61 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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