ABM99562
Protocadherin-11 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Protocadherin-11 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Protocadherin-11 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Protocadherin-11.
Background
This gene belongs to the protocadherin family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing seven cadherin repeats, a transmembrane domain, and a cytoplasmic tail that differs from those of the classical cadherins. This gene is located on the Y chromosome in a block of X/Y homology and is very closely related to its paralog on the X chromosome. The protein is thought to play a role in cell-cell recognition during development of the central nervous system. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013],
Additional antibody information
| Specificity | Protocadherin-11 Polyclonal Antibody detects endogenous levels of Protocadherin-11 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HepG2 cells, using PCDH-X/Y Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PCDH-X/Y Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: Protocadherin-11
| Target | Protocadherin-11 |
| Also known as | PCDH11Y; PCDH11; PCDH22; PCDHY; Protocadherin-11 Y-linked; Protocadherin-11; Protocadherin on the Y chromosome; PCDH-Y; Protocadherin prostate cancer; Protocadherin-PC; Protocadherin-22; PCDH11X; KIAA1326; PCDH11; PCDHX; Protocadherin-11 X- |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PCDH-X/Y. AA range:531-580 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Protocadherin-11
Also known as
PCDH11Y; PCDH11; PCDH22; PCDHY; Protocadherin-11 Y-linked; Protocadherin-11; Protocadherin on the Y chromosome; PCDH-Y; Protocadherin prostate cancer; Protocadherin-PC; Protocadherin-22; PCDH11X; KIAA1326; PCDH11; PCDHX; Protocadherin-11 X-
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
IHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PCDH-X/Y. AA range:531-580
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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