Nectin 4 rabbit pAb
Nectin 4 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Nectin 4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Nectin 4.
Background
This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011],
Additional antibody information
| Specificity | Nectin 4 Polyclonal Antibody detects endogenous levels of Nectin 4 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from NIH/3T3 cells, using PVRL4 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Nectin 4 |
| Also known as | PVRL4; LNIR; PRR4; Poliovirus receptor-related protein 4; Ig superfamily receptor LNIR; Nectin-4 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 55kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; Processed poliovirus receptor-related protein 4: Secreted . The secreted form is found in breast tumor patients (PubMed:15784625). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Adherens junction |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PVRL4. AA range:312-361 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.