CaMKIIα/δ (phospho Thr286) rabbit pAb from ELK Biotechnology Read more ›
CaMKIIα/δ (phospho Thr286) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CaMK2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008],
| Specificity | Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286. |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 (Phospho-Thr286) Antibody | Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7642 |
| Target | CaMK2 |
| Research areas | >>ErbB signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>HIF-1 signaling pathway, >>Oocyte meiosis, >>Necroptosis, >>Adrenergic signaling in cardiomyocytes, >>Wnt signaling pathway, >>Axon guidance, >>Circadian entrainment, >>Long-term potentiation, >>Neurotrophin signaling pathway, >>Cholinergic synapse, >>Dopaminergic synapse, >>Olfactory transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Glucagon signaling pathway, >>Aldosterone synthesis and secretion, >>Cushing syndrome, >>Gastric acid secretion, >>Parkinson disease, >>Pathways of neurodegeneration - multiple diseases, >>Amphetamine addiction, >>Tuberculosis, >>Pathways in cancer, >>Proteoglycans in cancer, >>Glioma, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Pig |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Pig |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Pig | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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