Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99484

COL14A1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

COL14A1 rabbit pAb from ELK Biotechnology Read more ›

COL14A1 rabbit pAb
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COL14A1 rabbit pAb

Product Description

COL14A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying COL14A1 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the alpha chain of type XIV collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XIV collagen interacts with the fibril surface and is involved in the regulation of fibrillogenesis. [provided by RefSeq, Jan 2013],

Additional antibody information

Specificity COL14A1 Polyclonal Antibody detects endogenous levels of COL14A1 protein.
Validation evidence captions Immunofluorescence analysis of HeLa cells, using Collagen XIV alpha1 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen XIV alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: COL14A1
Collaborator ELK Biotechnology
Partner model ES7478
Target COL14A1
Research areas>>Protein digestion and absorption
Reactivity Human,Mouse
Applications IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human N-ternal Collagen XIV alpha1. AA range:71-120
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Liver,Muscle,PNS,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
COL14A1
Also known as
COL14A1; UND; Collagen alpha-1(XIV) chain; Undulin
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
IHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human N-ternal Collagen XIV alpha1. AA range:71-120
Cellular localization
Secreted, extracellular space, extracellular matrix .
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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