mtTFA rabbit pAb from ELK Biotechnology Read more ›
mtTFA rabbit pAb is a research-use antibody from ELK Biotechnology for studying mtTFA in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
| Specificity | mtTFA Polyclonal Antibody detects endogenous levels of mtTFA protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,mtTFA Polyclonal Antibody(Green) was diluted at 1:200(4° overnight). Histone H3 (Tri Methyl Lys36) Monoclonal Antibody(Q12) (Red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western blot analysis of 293T 3T3 lysis using mtTFA antibody. Antibody was diluted at 1:500 | Immunofluorescence analysis of HepG2 cells, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using TFAM Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7375 |
| Target | mtTFA |
| Research areas | >>Apelin signaling pathway, >>Huntington disease |
| Reactivity | Human,Rat,Mouse,Bovine |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TFAM. AA range:131-180 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Lymphocyte,Pooled,Uterus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse | |||||||
| Bovine |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Bovine | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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