Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99444

TBX3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TBX3 rabbit pAb from ELK Biotechnology Read more ›

TBX3 rabbit pAb
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TBX3 rabbit pAb

Product Description

TBX3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TBX3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity TBX3 Polyclonal Antibody detects endogenous levels of TBX3 protein.
Validation evidence captions Western Blot analysis of KB cells using TBX3 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of mouse-kidney mouse-brain KB 293T lysis using TBX3 antibody. Antibody was diluted at 1:1000

Key Facts

Product typePrimary Antibody Target: TBX3
Collaborator ELK Biotechnology
Partner model ES7348
Target TBX3
Research areas>>Signaling pathways regulating pluripotency of stem cells
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human TBX3. AA range:301-350
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TBX3
Also known as
TBX3; T-box transcription factor TBX3; T-box protein 3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TBX3. AA range:301-350
Observed band
79kD
Cellular localization
Nucleus .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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