LKB1 (phospho Thr189) rabbit pAb from ELK Biotechnology Read more ›
LKB1 (phospho Thr189) rabbit pAb is a research-use antibody from ELK Biotechnology for studying LKB1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-LKB1 (T189) Polyclonal Antibody detects endogenous levels of LKB1 protein only when phosphorylated at T189. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-LKB1 (T189) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LKB1 (Phospho-Thr189) Antibody | Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Thr189) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7309 |
| Target | LKB1 |
| Research areas | >>FoxO signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Tight junction, >>Adipocytokine signaling pathway |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human LKB1 around the phosphorylation site of Thr189. AA range:155-204 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. Strongest expression in testis and fetal liver. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
