Stat1 (phospho Ser727) rabbit pAb from ELK Biotechnology Read more ›
Stat1 (phospho Ser727) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Stat1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Stat1 (S727) Polyclonal Antibody detects endogenous levels of Stat1 protein only when phosphorylated at S727. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 293 PC-3 HepG2-UV cells using Phospho-Stat1 (S727) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT1 (Phospho-Ser727) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7294 |
| Target | Stat1 |
| Research areas | >>Chemokine signaling pathway, >>Necroptosis, >>Osteoclast differentiation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Leishmaniasis, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Pancreatic cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Inflammatory bowel disease |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Ser727. AA range:694-743 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell,Brain,Retina,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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