Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99418

Sos 2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Sos 2 rabbit pAb from ELK Biotechnology Read more ›

Sos 2 rabbit pAb
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Sos 2 rabbit pAb

Product Description

Sos 2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Sos 2 in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a regulatory protein that is involved in the positive regulation of ras proteins. Mutations in this gene are associated with Noonan Syndrome-9. [provided by RefSeq, Jul 2016],

Additional antibody information

Specificity Sos 2 Polyclonal Antibody detects endogenous levels of Sos 2 protein.
Validation evidence captions Western blot analysis of SOS2 Antibody. The lane on the right is blocked with the SOS2 peptide. | Western blot analysis of the lysates from HT-29 cells using SOS2 antibody. | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: Sos 2
Collaborator ELK Biotechnology
Partner model ES7253
Target Sos 2
Research areas>>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Chemokine signaling pathway, >>FoxO signaling pathway, >>Phospholipase D signaling pathway, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Focal adhesion, >>Gap junction, >>JAK-STAT signaling pathway, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Thermogenesis, >>Neurotrophin signaling pathway, >>Regulation of actin cytoskeleton, >>Insulin signaling pathway, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Relaxin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Alcoholism, >>Hepatitis C, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Che
Reactivity Human,Rat,Mouse
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human SOS2. AA range:631-680
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Sos 2
Also known as
SOS2; Son of sevenless homolog 2; SOS-2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human SOS2. AA range:631-680
Observed band
150kD
Cellular localization
intracellular,cytosol,
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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