Primary Antibody ISO 9001|ABMIUM Verified™
ABM99412

PHACTR4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PHACTR4 rabbit pAb from ELK Biotechnology Read more ›

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PHACTR4 rabbit pAb
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PHACTR4 rabbit pAb

Product Description

PHACTR4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving PHACTR4.

Background

This gene encodes a member of the phosphatase and actin regulator (PHACTR) family. Other PHACTR family members have been shown to inhibit protein phosphatase 1 (PP1) activity, and the homolog of this gene in the mouse has been shown to interact with actin and PP1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity PHACTR4 Polyclonal Antibody detects endogenous levels of PHACTR4 protein.
Validation evidence captions Western blot analysis of PHACTR4 Antibody. The lane on the right is blocked with the PHACTR4 peptide. | Western blot analysis of the lysates from HeLa cells using PHACTR4 antibody. | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: PHACTR4
Target PHACTR4
Also known as PHACTR4; Phosphatase and actin regulator 4
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Species reactivity Human;Rat;Mouse;
Observed band 80kD
Cellular localization Cytoplasm . Cell projection, lamellipodium .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCELISA
Dilution range WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen The antiserum was produced against synthesized peptide derived from human PHACTR4. AA range:363-412
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
PHACTR4
Also known as
PHACTR4; Phosphatase and actin regulator 4
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PHACTR4. AA range:363-412
Observed band
80kD
Cellular localization
Cytoplasm . Cell projection, lamellipodium .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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