Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99404

GABA T-1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GABA T-1 rabbit pAb from ELK Biotechnology Read more ›

GABA T-1 rabbit pAb
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GABA T-1 rabbit pAb

Product Description

GABA T-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GABA T-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The SLC6A1 gene encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft (Hirunsatit et al., 2009 [PubMed 19077666]).[supplied by OMIM, Jul 2009],

Additional antibody information

Specificity GABA T-1 Polyclonal Antibody detects endogenous levels of GABA T-1 protein.
Validation evidence captions Western blot analysis of SLC6A1 Antibody. The lane on the right is blocked with the SLC6A1 peptide. | Western blot analysis of the lysates from HeLa cells using SLC6A1 antibody.

Key Facts

Product typePrimary Antibody Target: GABA T-1
Collaborator ELK Biotechnology
Partner model ES7218
Target GABA T-1
Research areas>>Synaptic vesicle cycle, >>GABAergic synapse
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human SLC6A1. AA range:381-430
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GABA T-1
Also known as
SLC6A1; GABATR; GABT1; GAT1; Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC6A1. AA range:381-430
Observed band
67kD
Cellular localization
Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Cell junction, synapse, presynapse . Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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