Primary Antibody ISO 9001|ABMIUM Verified™
ABM99403

SGLT-2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SGLT-2 rabbit pAb from ELK Biotechnology Read more ›

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SGLT-2 rabbit pAb
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SGLT-2 rabbit pAb

Product Description

SGLT-2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving SGLT-2.

Background

This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015],

Additional antibody information

Specificity SGLT-2 Polyclonal Antibody detects endogenous levels of SGLT-2 protein.
Validation evidence captions Western blot analysis of lysates from 293 cells, using SLC5A2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using SLC5A2 antibody. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: SGLT-2
Target SGLT-2
Also known as SLC5A2; SGLT2; Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300
Species reactivity Human;Mouse;Rat
Observed band 73kD
Cellular localization Apical cell membrane ; Multi-pass membrane protein .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHC
Dilution range WB 1:500-2000;IHC-p 1:50-300
Immunogen The antiserum was produced against synthesized peptide derived from human SLC5A2. AA range:101-150
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
SGLT-2
Also known as
SLC5A2; SGLT2; Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC5A2. AA range:101-150
Observed band
73kD
Cellular localization
Apical cell membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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