Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99397

Ran BP-17 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Ran BP-17 rabbit pAb from ELK Biotechnology Read more ›

Ran BP-17 rabbit pAb
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Ran BP-17 rabbit pAb

Product Description

Ran BP-17 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ran BP-17 in WB, IHC workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

RAN binding protein 17(RANBP17) Homo sapiens The transport of protein and large RNAs through the nuclear pore complexes (NPC) is an energy-dependent and regulated process. The import of proteins with a nuclear localization signal (NLS) is accomplished by recognition of one or more clusters of basic amino acids by the importin-alpha/beta complex; see MIM 600685 and MIM 602738. The small GTPase RAN (MIM 601179) plays a key role in NLS-dependent protein import. RAN-binding protein-17 is a member of the importin-beta superfamily of nuclear transport receptors.[supplied by OMIM, Jul 2002],

Additional antibody information

Specificity Ran BP-17 Polyclonal Antibody detects endogenous levels of Ran BP-17 protein.
Validation evidence captions Western blot analysis of RANBP17 Antibody. The lane on the right is blocked with the RANBP17 peptide. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: Ran BP-17
Collaborator ELK Biotechnology
Partner model ES7204
Target Ran BP-17
Research areas>>Nucleocytoplasmic transport
Reactivity Human,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human RANBP17. AA range:145-194
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in testis, moderately in pancreas and weakly in other tissues studied.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Ran BP-17
Also known as
RANBP17; Ran-binding protein 17
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human RANBP17. AA range:145-194
Observed band
125kD
Cellular localization
Cytoplasm . Nucleus . Nucleus, nuclear pore complex .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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