Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99389

SH3BGR rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SH3BGR rabbit pAb from ELK Biotechnology Read more ›

SH3BGR rabbit pAb
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SH3BGR rabbit pAb

Product Description

SH3BGR rabbit pAb is a research-use antibody from ELK Biotechnology for studying SH3BGR in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

caution:It is uncertain whether Met-1 or Met-64 is the initiator.,similarity:Belongs to the SH3BGR family.,tissue specificity:Expressed in heart and skeletal muscle.,

Additional antibody information

Specificity SH3BGR Polyclonal Antibody detects endogenous levels of SH3BGR protein.
Validation evidence captions Western blot analysis of lysates from Jurkat cells, using SH3BGR Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: SH3BGR
Collaborator ELK Biotechnology
Partner model ES7183
Target SH3BGR
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human SH3BGR. AA range:81-130
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in heart and skeletal muscle.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
SH3BGR
Also known as
SH3BGR; SH3 domain-binding glutamic acid-rich protein; SH3BGR protein; 21-glutamic acid-rich protein; 21-GARP
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human SH3BGR. AA range:81-130
Observed band
26kD
Cellular localization
cytosol,
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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