Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99386

Nkx-2.4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Nkx-2.4 rabbit pAb from ELK Biotechnology Read more ›

Nkx-2.4 rabbit pAb
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Nkx-2.4 rabbit pAb

Product Description

Nkx-2.4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nkx-2.4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Probable transcription factor.,similarity:Belongs to the NK-2 homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,

Additional antibody information

Specificity NKX2.4 Polyclonal Antibody detects endogenous levels of NKX2.4 protein.
Validation evidence captions Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: Nkx-2.4
Collaborator ELK Biotechnology
Partner model ES7178
Target Nkx-2.4
Research areasAntibody Research
Reactivity Human,Mouse,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Nkx-2.4 . at AA range: 250-330
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Nkx-2.4
Also known as
NKX2-4; NKX2D; Homeobox protein Nkx-2.4; Homeobox protein NK-2 homolog D
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Monkey
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Nkx-2.4 . at AA range: 250-330
Observed band
36kD
Cellular localization
Nucleus .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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