Primary Antibody ISO 9001|ABMIUM Verified™
ABM99369

GFRα-4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GFRα-4 rabbit pAb from ELK Biotechnology Read more ›

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GFRα-4 rabbit pAb
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GFRα-4 rabbit pAb

Product Description

GFRα-4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving GFRα-4.

Background

The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for RET-associated diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity GFRα-4 Polyclonal Antibody detects endogenous levels of GFRα-4 protein.
Validation evidence captions Western blot analysis of lysates from HeLa cells, using GFRA4 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product type Primary Antibody Target: GFRα-4
Target GFRα-4
Also known as GFRA4; GDNF family receptor alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR-alpha-4; Persephin receptor
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Species reactivity Human;Rat;Mouse;
Observed band 30kD
Cellular localization Isoform GFRalpha4a: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform GFRalpha4b: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform GFRalpha4c: Secreted.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from human GFRA4. AA range:141-190
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GFRα-4
Also known as
GFRA4; GDNF family receptor alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR-alpha-4; Persephin receptor
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GFRA4. AA range:141-190
Observed band
30kD
Cellular localization
Isoform GFRalpha4a: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform GFRalpha4b: Cell membrane; Lipid-anchor, GPI-anchor.; Isoform GFRalpha4c: Secreted.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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