Syndecan-4 rabbit pAb from ELK Biotechnology Read more ›
Syndecan-4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Syndecan-4 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008],
| Specificity | Syndecan-4 Polyclonal Antibody detects endogenous levels of Syndecan-4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HepG2 cells using Syndecan-4 Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using Syndecan4 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Syndecan4 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 cells, using Syndecan4 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7137 |
| Target | Syndecan-4 |
| Research areas | >>ECM-receptor interaction, >>Cell adhesion molecules, >>Proteoglycans in cancer, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Syndecan4. AA range:145-194 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in epithelial and fibroblastic cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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