ENaCδ rabbit pAb from ELK Biotechnology Read more ›
ENaCδ rabbit pAb is a research-use antibody from ELK Biotechnology for studying ENaCδ in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.,similarity:Belongs to the amiloride-sensitive sodium channel family.,subunit:Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit.,
| Specificity | ENaCδ Polyclonal Antibody detects endogenous levels of ENaCδ protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using SCNN1D Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using SCNN1D Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using SCNN1D antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7127 |
| Target | ENaCδ |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SCNN1D. AA range:411-460 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Hippocampus,Lung,Nasal epithelium,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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