Bradykinin B1 R rabbit pAb
Bradykinin B1 R rabbit pAb from ELK Biotechnology Read more ›
Product Description
Bradykinin B1 R rabbit pAb is an ELK Biotechnology antibody for research workflows involving Bradykinin B1 R.
Background
Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Additional antibody information
| Specificity | Bradykinin B1 R Polyclonal Antibody detects endogenous levels of Bradykinin B1 R protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of LOVO cells, using BDKRB1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using BDKRB1 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Bradykinin B1 R |
| Also known as | BDKRB1; BRADYB1; B1 bradykinin receptor; B1R; BK-1 receptor |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 40kD |
| Cellular localization | Cell membrane; Multi-pass membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Calcium signaling pathway;>>Neuroactive ligand-receptor interaction;>>Complement and coagulation cascades;>>Inflammatory mediator regulation of TRP channels;>>Regulation of actin cytoskeleton;>>Pathways in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BDKRB1. AA range:201-250 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.