p70 S6 kinase β (phospho Ser423) rabbit pAb from ELK Biotechnology Read more ›
p70 S6 kinase β (phospho Ser423) rabbit pAb is a research-use antibody from ELK Biotechnology for studying P70S6k in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
ribosomal protein S6 kinase B2(RPS6KB2) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015],
| Specificity | Phospho-p70 S6 kinase β (S423) Polyclonal Antibody detects endogenous levels of p70 S6 kinase β protein only when phosphorylated at S423. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 5', using p70 S6 Kinase beta (Phospho-Ser423) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7097 |
| Target | P70S6k |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>ErbB signaling pathway, >>HIF-1 signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>TGF-beta signaling pathway, >>Apelin signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Thermogenesis, >>Insulin signaling pathway, >>Insulin resistance, >>Shigellosis, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Chemical carcinogenesis - receptor activation, >>Colorectal cancer, >>Pancreatic cancer, >>Acute myeloid leukemia, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer, >>Choline metabolism in cancer, >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase beta around the phosphorylation site of Ser423. AA range:389-438 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium,Lymph, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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