Ribosomal Protein S6 (phospho Ser235) rabbit pAb
Ribosomal Protein S6 (phospho Ser235) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Ribosomal Protein S6 (phospho Ser235) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Ribosomal Protein S6.
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed
Additional antibody information
| Specificity | Phospho-Ribosomal Protein S6 (S235) Polyclonal Antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at S235. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S6 Ribosomal Protein (Phospho-Ser235) Antibody | Western blot analysis of lysates from 293 cells treated with serum 10% 15', using S6 Ribosomal Protein (Phospho-Ser235) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | Ribosomal Protein S6 |
| Also known as | RPS6; OK/SW-cl.2; 40S ribosomal protein S6; Phosphoprotein NP33 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 32kD |
| Cellular localization | nucleus,nucleoplasm,nucleolus,cytoplasm,cytosol,ribosome,polysome,small ribosomal subunit,membrane,cytosolic small ribosomal subunit,dendrite,intracellular ribonucleoprotein complex,cytoplasmic ribonucleoprotein granu |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>Ribosome;>>HIF-1 signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apelin signaling pathway;>>Thermogenesis;>>Insulin signaling pathway;>>Coronavirus disease - COVID-19;>>Proteoglycans in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser235. AA range:200-249 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.