RGS7 rabbit pAb
RGS7 rabbit pAb from ELK Biotechnology Read more ›
Product Description
RGS7 rabbit pAb is an ELK Biotechnology antibody for research workflows involving RGS7.
Background
function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(o)-alpha is specifically enhanced by the RGS6/GNG5 dimer. May play a role in synaptic vesicle exocytosis. May play important role in the rapid regulation of neuronal excitability and the cellular responses to short-lived stimulations.,PTM:Palmitoylated.,PTM:Phosphorylation and subsequent interaction with 14-3-3 proteins inhibits GAP activity.,similarity:Contains 1 DEP domain.,similarity:Contains 1 G protein gamma domain.,similarity:Contains 1 RGS domain.,subunit:Heterodimer with GNG5. Interacts with RGS7BP, leading to regulate the subcellular location of the heterodimer formed with Gbeta5 (By similarity). Interacts with 14-3-3 protein Tau and SNAP25BP.,
Additional antibody information
| Specificity | RGS7 Polyclonal Antibody detects endogenous levels of RGS7 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat cells, using RGS7 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | RGS7 |
| Also known as | RGS7; Regulator of G-protein signaling 7; RGS7 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 65kD |
| Cellular localization | Cytoplasm, cytosol . Cytoplasm . Cell membrane . Membrane ; Peripheral membrane protein ; Cytoplasmic side . Interaction with PKD1 promotes location at the cell membrane (PubMed:10339594). Interaction with RGS7BP promotes location at the cell membrane (PubMed:15897264). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RGS7. AA range:155-204 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.