Bcl-x (phospho Thr47) rabbit pAb from ELK Biotechnology Read more ›
Bcl-x (phospho Thr47) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Bcl-x in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015],
| Specificity | Phospho-Bcl-x (T47) Polyclonal Antibody detects endogenous levels of Bcl-x protein only when phosphorylated at T47. |
|---|---|
| Validation evidence captions | Western blot analysis of RAT-MUSLE using Phospho-Bcl-x (T47) antibody. Antibody was diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BCL-XL (Phospho-Thr47) Antibody | Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES7035 |
| Target | Bcl-x |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Platinum drug resistance, >>Ras signaling pathway, >>NF-kappa B signaling pathway, >>p53 signaling pathway, >>Mitophagy - animal, >>Autophagy - animal, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Apoptosis - multiple species, >>NOD-like receptor signaling pathway, >>JAK-STAT signaling pathway, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Toxoplasmosis, >>Measles, >>Human T-cell leukemia virus 1 infection, >>Herpes simplex virus 1 infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Pancreatic cancer, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Hepatocellular carcinoma, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BCL-XL around the phosphorylation site of Thr47. AA range:13-62 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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