ABM99309
MYL7 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
MYL7 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
MYL7 rabbit pAb is an ELK Biotechnology antibody for research workflows involving MYL7.
Background
miscellaneous:This chain binds calcium.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Predominantly expressed in adult atrial muscle.,
Additional antibody information
| Specificity | MYL7 Polyclonal Antibody detects endogenous levels of MYL7 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: MYL7
| Target | MYL7 |
| Also known as | MYL7; MYL2A; MYLC2A; Myosin regulatory light chain 2; atrial isoform; MLC-2a; MLC2a; Myosin light chain 2a; Myosin regulatory light chain 7 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse;Rat;Monkey |
| Observed band | 18kD |
| Cellular localization | cytosol,myosin complex,A band,dendritic spine, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Focal adhesion;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Shigellosis;>>Salmonella infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYL7. AA range:111-160 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
MYL7
Also known as
MYL7; MYL2A; MYLC2A; Myosin regulatory light chain 2; atrial isoform; MLC-2a; MLC2a; Myosin light chain 2a; Myosin regulatory light chain 7
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat;Monkey
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human MYL7. AA range:111-160
Observed band
18kD
Cellular localization
cytosol,myosin complex,A band,dendritic spine,
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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