Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99309

MYL7 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MYL7 rabbit pAb from ELK Biotechnology Read more ›

MYL7 rabbit pAb
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MYL7 rabbit pAb

Product Description

MYL7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MYL7 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

miscellaneous:This chain binds calcium.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,tissue specificity:Predominantly expressed in adult atrial muscle.,

Additional antibody information

Specificity MYL7 Polyclonal Antibody detects endogenous levels of MYL7 protein.
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: MYL7
Collaborator ELK Biotechnology
Partner model ES6961
Target MYL7
Research areas>>Focal adhesion, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Shigellosis, >>Salmonella infection
Reactivity Human,Mouse,Rat,Monkey
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human MYL7. AA range:111-160
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Predominantly expressed in adult atrial muscle.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
Monkey
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
MYL7
Also known as
MYL7; MYL2A; MYLC2A; Myosin regulatory light chain 2; atrial isoform; MLC-2a; MLC2a; Myosin light chain 2a; Myosin regulatory light chain 7
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human MYL7. AA range:111-160
Observed band
18kD
Cellular localization
cytosol,myosin complex,A band,dendritic spine,
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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