PTP1B (phospho Ser50) rabbit pAb from ELK Biotechnology Read more ›
PTP1B (phospho Ser50) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PTP1B in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of
| Specificity | Phospho-PTP1B (S50) Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at S50. |
|---|---|
| Validation evidence captions | Western Blot analysis of COLO cells using Phospho-PTP1B (S50) Polyclonal Antibody diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PTP1B (Phospho-Ser50) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PTP1B (Phospho-Ser50) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COS7 cells treated with UV 30', using PTP1B (Phospho-Ser50) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6929 |
| Target | PTP1B |
| Research areas | >>Adherens junction, >>Insulin signaling pathway, >>Insulin resistance, >>Chemical carcinogenesis - reactive oxygen species |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PTP1B around the phosphorylation site of Ser50. AA range:16-65 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in keratinocytes (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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