Manufactured by:ELK Biotechnology
RUO

FAK (phospho Ser910) rabbit pAb

FAK (phospho Ser910) rabbit pAb from ELK Biotechnology

SKU:
ABM109145
Product type
Primary Antibody
TargetFAK
ApplicationsIHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemDetected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous. Expressed in epithelial cells (at protein level) (PubMed:31630787).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

FAK (phospho Ser910) rabbit pAb is a research-use antibody from ELK Biotechnology for studying FAK in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

protein tyrosine kinase 2(PTK2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015],

Additional antibody information

Specificity Phospho-FAK (S910) Polyclonal Antibody detects endogenous levels of FAK protein only when phosphorylated at S910.
Validation evidence captions Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Endocrine resistance, >>ErbB signaling pathway, >>Chemokine signaling pathway, >>PI3K-Akt signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Focal adhesion, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Growth hormone synthesis, secretion and action, >>Bacterial invasion of epithelial cells, >>Shigellosis, >>Yersinia infection, >>Amoebiasis, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Proteoglycans in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Small cell lung cancer, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis
Also known as
PTK2; FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Ser910. AA range:876-925
Cellular localization
Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus. Cytoplasm, cytoskeleton, cilium basal body . Constituent of focal adhesions. Detected at microtubules.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
119kD
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 876-925
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES6905
UniProt entry
Q05397
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00