Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99276

CYP20A1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CYP20A1 rabbit pAb from ELK Biotechnology Read more ›

CYP20A1 rabbit pAb
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CYP20A1 rabbit pAb

Product Description

CYP20A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP20A1 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

cytochrome P450 family 20 subfamily A member 1(CYP20A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CYP20A1 Polyclonal Antibody detects endogenous levels of CYP20A1 protein.
Validation evidence captions Western blot analysis of Cytochrome P450 20A1 Antibody. The lane on the right is blocked with the Cytochrome P450 20A1 peptide. | Western blot analysis of the lysates from HUVECcells using Cytochrome P450 20A1 antibody.

Key Facts

Product typePrimary Antibody Target: CYP20A1
Collaborator ELK Biotechnology
Partner model ES6893
Target CYP20A1
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human Cytochrome P450 20A1. AA range:191-240
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Blood,Lung,Pheochromocytoma,Placenta,Stomach,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CYP20A1
Also known as
CYP20A1; Cytochrome P450 20A1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 20A1. AA range:191-240
Observed band
53kD
Cellular localization
Membrane ; Single-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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