MEK-7 (phospho Ser271) rabbit pAb from ELK Biotechnology Read more ›
MEK-7 (phospho Ser271) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MKK7 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
| Specificity | Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MAP2K7 (Phospho-Ser271) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH/3T3 cells treated with insulin 0.01U/ml 15' and NIH/3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6833 |
| Target | MKK7 |
| Research areas | >>MAPK signaling pathway, >>ErbB signaling pathway, >>Protein processing in endoplasmic reticulum, >>Osteoclast differentiation, >>Tight junction, >>Toll-like receptor signaling pathway, >>T cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>GnRH signaling pathway, >>Relaxin signaling pathway, >>Alcoholic liver disease, >>Alzheimer disease, >>Huntington disease, >>Pathways of neurodegeneration - multiple diseases, >>Salmonella infection, >>Yersinia infection, >>Hepatitis B, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Human immunodeficiency virus 1 infection, >>Chemical carcinogenesis - reactive oxygen species, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range:236-285 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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