Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99249

ApoER2 Antibody – ApoER2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ApoER2 rabbit pAb from ELK Biotechnology Read more ›

ApoER2 rabbit pAb
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ApoER2 rabbit pAb

Product Description

ApoER2 Antibody for research use. Review the supplied specifications, applications and documentation before ordering.

ApoER2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ApoER2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2011],

Additional antibody information

Specificity ApoER2 Polyclonal Antibody detects endogenous levels of ApoER2 protein.
Validation evidence captions Western blot analysis of the lysates from HeLa cells using LRP8 antibody.

How to evaluate ApoER2 Antibody – ApoER2 rabbit pAb

Assess this research-use product against the requirements of your experimental workflow before ordering.

  • Confirm the listed species reactivity and experimental application.
  • Review clonality, host species, format, concentration and storage conditions.
  • Use lot-specific documentation and suitable positive and negative controls.

If a specification or current document is not shown, contact ABMIUM scientific support for assistance before use.

Key Facts

Product typePrimary Antibody Target: ApoER2
Collaborator ELK Biotechnology
Partner model ES6810
Target ApoER2
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human LRP8. AA range:451-500
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ApoER2
Also known as
LRP8; APOER2; Low-density lipoprotein receptor-related protein 8; LRP-8; Apolipoprotein E receptor 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human LRP8. AA range:451-500
Observed band
100kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Secreted . Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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