PKC ζ (phospho Thr560) rabbit pAb from ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
PKC ζ (phospho Thr560) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PKC ζ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-PKC ζ (T560) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T560. |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
