PKD1/2/3 (phospho Ser738/S742) rabbit pAb from ELK Biotechnology Read more ›
PKD1/2/3 (phospho Ser738/S742) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PKD1/2/3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],
| Specificity | Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody detects endogenous levels of PKD1/2/3 protein only when phosphorylated at S738/S742. |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 cells using Phospho-PKD1/2/3 (S738/S742) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody | Immunohistochemistry analysis of paraffin-embedded human heart, using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30' and HT29 cells treated with serum 20% 15', using PKD1/2/3/PKC mu (Phospho-Ser738+Ser742) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6789 |
| Target | PKD1/2/3 |
| Research areas | >>Rap1 signaling pathway, >>Aldosterone synthesis and secretion, >>Chemical carcinogenesis - reactive oxygen species |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKD1/2/3/PKC mu around the phosphorylation site of Ser738 and Ser742. AA range:706-755 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Placenta,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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