PKC δ (phospho Tyr64) rabbit pAb
PKC δ (phospho Tyr64) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PKC δ (phospho Tyr64) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PKC δ.
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-PKC δ (Y64) Polyclonal Antibody detects endogenous levels of PKC δ protein only when phosphorylated at Y64. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKCD (Phospho-Tyr64) Antibody | Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using PKCD (Phospho-Tyr64) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | PKC δ |
| Also known as | PRKCD; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 77kD |
| Cellular localization | Cytoplasm . Cytoplasm, perinuclear region . Nucleus . Cell membrane ; Peripheral membrane protein . Mitochondrion . Endomembrane system . Translocates to the mitochondria upon apoptotic stimulation. Upon activation, translocates to the plasma membrane followed by partial location to the endolysosomes (PubMed:17303575). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Chemokine signaling pathway;>>Autophagy - animal;>>Vascular smooth muscle contraction;>>NOD-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>Fc gamma R-mediated phagocytosis;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Estrogen signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Prion disease;>>Shigellosis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKCD around the phosphorylation site of Tyr64. AA range:30-79 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.