PKC α (phospho Thr638) rabbit pAb from ELK Biotechnology Read more ›
PKC α (phospho Thr638) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PKC α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
| Specificity | Phospho-PKC α (T638) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at T638. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-PKC α (T638) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC alpha (Phospho-Thr638) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC alpha (Phospho-Thr638) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using PKC alpha (Phospho-Thr638) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6762 |
| Target | PKC α |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>HIF-1 signaling pathway, >>Phosphatidylinositol signaling system, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Wnt signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Focal adhesion, >>Gap junction, >>Neutrophil extracellular trap formation, >>Natural killer cell mediated cytotoxicity, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Circadian entrainment, >>Long-term potentiation, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Inflammatory mediator regulation of TRP channe |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638. AA range:606-655 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Blood,Brain,Epithelium,Lung,Platelet, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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