Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99229

GPRC5D rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GPRC5D rabbit pAb from ELK Biotechnology Read more ›

GPRC5D rabbit pAb 50µL/100µL
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GPRC5D rabbit pAb 50µL/100µL

Product Description

GPRC5D rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPRC5D in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity GPRC5D Polyclonal Antibody detects endogenous levels of GPRC5D protein.
Validation evidence captions Immunofluorescence analysis of MCF7 cells, using GPRC5D Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat cells, using GPRC5D Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using GPRC5D antibody.

Key Facts

Product typePrimary Antibody Target: GPRC5D
Collaborator ELK Biotechnology
Partner model ES6729
Target GPRC5D
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GPRC5D. AA range:271-320
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GPRC5D
Also known as
GPRC5D; G-protein coupled receptor family C group 5 member D
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GPRC5D. AA range:271-320
Observed band
43kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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