AVP Receptor V2 rabbit pAb from ELK Biotechnology Read more ›
AVP Receptor V2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AVP Receptor V2 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus
| Specificity | AVP Receptor V2 Polyclonal Antibody detects endogenous levels of AVP Receptor V2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of MCF7 cells, using AVPR2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using AVPR2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using AVPR2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6726 |
| Target | AVP Receptor V2 |
| Research areas | >>Phospholipase D signaling pathway, >>Neuroactive ligand-receptor interaction, >>Vasopressin-regulated water reabsorption |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AVPR2. AA range:72-121 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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